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Brain-wide circuit interrogation at the cellular level guided by online analysis of neuronal function

Item Type:Article
Title:Brain-wide circuit interrogation at the cellular level guided by online analysis of neuronal function
Creators Name:Vladimirov, N. and Wang, C. and Höckendorf, B. and Pujala, A. and Tanimoto, M. and Mu, Y. and Yang, C.T. and Wittenbach, J.D. and Freeman, J. and Preibisch, S. and Koyama, M. and Keller, P.J. and Ahrens, M.B.
Abstract:Whole-brain imaging allows for comprehensive functional mapping of distributed neural pathways, but neuronal perturbation experiments are usually limited to targeting predefined regions or genetically identifiable cell types. To complement whole-brain measures of activity with brain-wide manipulations for testing causal interactions, we introduce a system that uses measured activity patterns to guide optical perturbations of any subset of neurons in the same fictively behaving larval zebrafish. First, a light-sheet microscope collects whole-brain data that are rapidly analyzed by a distributed computing system to generate functional brain maps. On the basis of these maps, the experimenter can then optically ablate neurons and image activity changes across the brain. We applied this method to characterize contributions of behaviorally tuned populations to the optomotor response. We extended the system to optogenetically stimulate arbitrary subsets of neurons during whole-brain imaging. These open-source methods enable delineating the contributions of neurons to brain-wide circuit dynamics and behavior in individual animals.
Keywords:Animal Behavior, Brain, Brain Mapping, Larva, Neural Pathways, Neurons, Online Systems, Swimming, Animals, Zebrafish
Source:Nature Methods
Publisher:Nature Publishing Group
Page Range:1117-1125
Date:December 2018
Official Publication:https://doi.org/10.1038/s41592-018-0221-x
PubMed:View item in PubMed

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