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Imaging of endothelial cell dynamic behavior in zebrafish

Item Type:Article
Title:Imaging of endothelial cell dynamic behavior in zebrafish
Creators Name:Coxam, B. and Gerhardt, H.
Abstract:In recent years, use of the zebrafish embryo as a model organism to study vascular development in vivo has provided valuable insights into the genetic and cellular events shaping the embryonic vasculature. In this chapter, we aim to present the methods for the measurement of some of the most commonly investigated dynamic parameters in endothelial cells during developmental angiogenesis, namely, migration speed and acceleration, filopodia extension, front-rear polarity, cell cycle progression, membrane deformations, and junctional rearrangements. We also offer suggestions on how to deal with the most common imaging and quantifications challenges faced when acquiring and quantifying endothelial cell dynamic behavior in vivo.We intend this section to serve as an experience-based imaging primer for scientists interested in endothelial cell imaging in the zebrafish embryo.
Keywords:Endothelial Cells, Spinning Disk, Kymograph, Polarity, Membrane, Cell Cycle, Vasculature, Animals, Zebrafish
Source:Methods in Molecular Biology
Title of Book:Lymphangiogenesis: Methods and Protocols
ISSN:1064-3745
ISBN:978-1-4939-8711-5
Publisher:Springer / Humana Press (U.S.A.)
Volume:1846
Page Range:181-195
Date:2018
Official Publication:https://doi.org/10.1007/978-1-4939-8712-2_12
PubMed:View item in PubMed

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