Item Type: | Article |
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Title: | In vivo imaging of MMP-13 activity using a specific polymer-FRET peptide conjugate detects early osteoarthritis and inhibitor efficacy |
Creators Name: | Duro-Castano, A. and Lim, N.H. and Tranchant, I. and Amoura, M. and Beau, F. and Wieland, H. and Kingler, O. and Herrmann, M. and Nazaré, M. and Plettenburg, O. and Dive, V. and Vicent, M.J. and Nagase, H. |
Abstract: | Imaging early molecular changes in osteoarthritic (OA) joints is instrumental for the development of disease‐modifying drugs. To this end, a fluorescent resonance energy transfer-based peptide probe that is cleavable by matrix metalloproteinase 13 (MMP-13) has been developed. This protease degrades type II collagen, a major matrix component of cartilage. The probe exhibits high catalytic efficiency (kcat/KM = 6.5 × 105m-1 s-1) and high selectivity for MMP-13 over a set of nine MMPs. To achieve optimal in vivo pharmacokinetics and tissue penetration, the probe has been further conjugated to a linear l-polyglutamate chain of 30 kDa. The conjugate detects early biochemical events that occur in a surgically induced murine model of OA before major histological changes. The nanometric probe is suitable for the monitoring of in vivo efficacy of an orally bioavailable MMP-13 inhibitor, which effectively blocks cartilage degradation during the development of OA. This new polymer-probe can therefore be a useful tool in detecting early OA, disease progression, and in developing MMP-13-based disease-modifying drugs for OA. |
Keywords: | Early Detection, Live Imaging, MMP‐13 Nanoprobes, Osteoarthritis, Polymer Therapeutics, Animals, Mice |
Source: | Advanced Functional Materials |
ISSN: | 1616-301X |
Publisher: | Wiley-VCH |
Volume: | 28 |
Number: | 37 |
Page Range: | 1802738 |
Date: | 12 September 2018 |
Official Publication: | https://doi.org/10.1002/adfm.201802738 |
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