Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Human axial progenitors generate trunk neural crest cells in vitro

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
5MB

Item Type:Article
Title:Human axial progenitors generate trunk neural crest cells in vitro
Creators Name:Frith, T.J.R. and Granata, I. and Wind, M. and Stout, E. and Thompson, O. and Neumann, K. and Stavish, D. and Heath, P.R. and Ortmann, D. and Hackland, J.O.S. and Anastassiadis, K. and Gouti, M. and Briscoe, J. and Wilson, V. and Johnson, S.L. and Placzek, M. and Guarracino, M.R. and Andrews, P.W. and Tsakiridis, A.
Abstract:The neural crest (NC) is a multipotent embryonic cell population that generates distinct cell types in an axial position-dependent manner. The production of NC cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology. However, the origin of human trunk NC remains undefined and current in vitro differentiation strategies induce only a modest yield of trunk NC cells. Here we show that hPSC-derived axial progenitors, the posteriorly-located drivers of embryonic axis elongation, give rise to trunk NC cells and their derivatives. Moreover, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities in vitro. Collectively, our findings indicate that there are two routes toward a human post-cranial NC state: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas trunk NC arises within a pool of posterior axial progenitors.
Keywords:Biomarkers, Cell Differentiation, Cultured Cells, Neural Crest, Pluripotent Stem Cells
Source:eLife
ISSN:2050-084X
Publisher:eLife Sciences Publications (U.K.)
Volume:7
Page Range:e35786
Date:10 August 2018
Official Publication:https://doi.org/10.7554/eLife.35786
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library