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Interleukin 10 transfected into Chinese hamster ovary cells prevents tumor growth and macrophage infiltration

Item Type:Article
Title:Interleukin 10 transfected into Chinese hamster ovary cells prevents tumor growth and macrophage infiltration
Creators Name:Richter, G. and Krueger-Krasagakes, S. and Hein, G. and Huels, C. and Schmitt, E. and Diamantstein, T. and Blankenstein, T.
Abstract:Expression of cytokines in tumor cells provides a sensitive modality to analyze the consequences of local cytokines in vivo on tumor infiltrating cells and tumorigenicity. We have transfected Chinese hamster ovary (CHO) cells with an interleukin 10 (IL-10) expression vector. CHO-IL10 cells although unaltered with respect to their in vitro growth lost tumorigenicity, both in nude and in SCID mice and in an IL-10 dose dependent manner. In addition, CHO-IL10 cells suppressed the growth of equal numbers of coinjected but not of contralaterally injected CHO cells. Immunohistology with anti-CR3/Mac-1 and anti-Mac-3 monoclonal antibodies revealed that CHO tumors were substantially infiltrated by macrophages. However, in CHO-IL10 tumors macrophages were virtually absent within the tumor tissue. Our results suggest that IL-10 indirectly suppresses tumor growth of certain tumors by inhibiting infiltration of macrophages which may provide tumor growth promoting activity.
Keywords:Base Sequence, CHO Cells, Experimental Neoplasms, Gene Therapy, Interleukin-10, Macrophages, Molecular Sequence Data, Transfection, Animals, Cricetinae, Mice
Source:Cancer Research
ISSN:0008-5472
Publisher:American Association for Cancer Research (U.S.A.)
Volume:53
Number:18
Page Range:4134-4137
Date:15 September 1993
Official Publication:http://cancerres.aacrjournals.org/content/53/18/4134.abstract
PubMed:View item in PubMed

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