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Serial interactome capture of the human cell nucleus

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Item Type:Article
Title:Serial interactome capture of the human cell nucleus
Creators Name:Conrad, T. and Albrecht, A.-S. and Rodrigues de Melo Costa, V. and Sauer, S. and Meierhofer, D. and Ørom, U.A.
Abstract:Novel RNA-guided cellular functions are paralleled by an increasing number of RNA-binding proteins (RBPs). Here we present 'serial RNA interactome capture' (serIC), a multiple purification procedure of ultraviolet-crosslinked poly(A)-RNA-protein complexes that enables global RBP detection with high specificity. We apply serIC to the nuclei of proliferating K562 cells to obtain the first human nuclear RNA interactome. The domain composition of the 382 identified nuclear RBPs markedly differs from previous IC experiments, including few factors without known RNA-binding domains that are in good agreement with computationally predicted RNA binding. serIC extends the number of DNA-RNA-binding proteins (DRBPs), and reveals a network of RBPs involved in p53 signalling and double-strand break repair. serIC is an effective tool to couple global RBP capture with additional selection or labelling steps for specific detection of highly purified RBPs.
Keywords:Cell Nucleus, DNA, DNA-Binding Proteins, Gene Expression Regulation, Gene Regulatory Networks, Guide RNA, HEK293 Cells, HeLa Cells, K562 Cells, Messenger RNA, Protein Interaction Mapping, RNA-Binding Proteins, Signal Transduction, Transcription Factors, Ultraviolet Rays
Source:Nature Communications
ISSN:2041-1723
Publisher:Nature Publishing Group (U.K.)
Volume:7
Page Range:11212
Date:4 April 2016
Official Publication:https://doi.org/10.1038/ncomms11212
PubMed:View item in PubMed

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