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C/EBP beta regulation of the tumor necrosis factor alpha gene

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Item Type:Article
Title:C/EBP beta regulation of the tumor necrosis factor alpha gene
Creators Name:Pope, R.M. and Leutz, A. and Ness, S.A.
Abstract:Activated macrophages contribute to chronic inflammation by the secretion of cytokines and proteinases. Tumor necrosis factor alpha (TNF alpha) is particularly important in this process because of its ability to regulate other inflammatory mediators in an autocrine and paracrine fashion. The mechanism(s) responsible for the cell type-specific regulation of TNF alpha is not known. We present data to show that the expression of TNF alpha is regulated by the transcription factor C/EBP beta (NF-IL6). C/EBP beta activated the TNF alpha gene promoter in cotransfection assays and bound to it at a site which failed to bind the closely related protein C/EBP alpha. Finally, a dominant-negative version of C/EBP beta blocked TNF alpha promoter activation in myeloid cells. Our results implicate C/EBP beta as an important regulator of TNF alpha by myelomonocytic cells.
Keywords:Base Sequence, Binding Sites, CCAAT-Enhancer-Binding Proteins, Cell Line, DNA, DNA-Binding Proteins, Molecular Sequence Data, Monocytes, Mutation, Nuclear Proteins, Promoter Regions (Genetics), Recombinant Fusion Proteins, T-Lymphocytes, Tetradecanoylphorbol Acetate, Trans-Activation (Genetics), Transcription Factors, Transfection, Cultured Tumor Cells, Tumor Necrosis Factor-alpha
Source:Journal of Clinical Investigation
ISSN:0021-9738
Publisher:American Society for Clinical Investigation (U.S.A.)
Volume:94
Number:4
Page Range:1449-1455
Date:October 1994
Official Publication:https://doi.org/10.1172/JCI117482
PubMed:View item in PubMed

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