PolNet Analysis: a software tool for the quantification of network-level endothelial cell polarity and blood flow during vascular remodelling
| Item Type: | Preprint |
|---|
| Title: | PolNet Analysis: a software tool for the quantification of network-level endothelial cell polarity and blood flow during vascular remodelling |
|---|
| Creators Name: | Bernabeu, M.O. and Jones, M.L. and Nash, R.W. and Pezzarossa, A. and Coveney, P.V. and Gerhardt, H. and Franco, C.A. |
|---|
| Abstract: | In this paper, we present PolNet, an open source software tool for the study of blood flow and cell-level biological activity during vessel morphogenesis. We provide an image acquisition, segmentation, and analysis protocol to quantify endothelial cell polarity in entire in vivo vascular networks. In combination, we use computational fluid dynamics to characterise the haemodynamics of the vascular networks under study. The tool enables, for the first time, network-level analysis of polarity and flow for individual endothelial cells. To date, PolNet has proven invaluable for the study of endothelial cell polarisation and migration during vascular patterning, as demonstrated by our recent papers (Franco 2015, Franco 2016a). Additionally, the tool can be easily extended to correlate blood flow with other experimental observations at the cellular/molecular level. We release the source code of our tool under the LGPL licence. |
|---|
| Source: | bioRxiv |
|---|
| Publisher: | Cold Spring Harbor Laboratory Press |
|---|
| Article Number: | 237602 |
|---|
| Date: | 22 December 2017 |
|---|
| Official Publication: | https://doi.org/10.1101/237602 |
|---|
| Related to: | |
|---|
Repository Staff Only: item control page
Downloads per month over past year
|
![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Download Statistics
Download Statistics
