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Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types

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Item Type:Article
Title:Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types
Creators Name:Ness, S.A. and Kowenz-Leutz, E. and Casini, T. and Graf, T. and Leutz, A.
Abstract:The c-Myb transcription factor regulates the differentiation of immature erythroid, lymphoid, and myeloid cells, although only the latter cells become transformed by the v-myb oncogene. These are also the only cells that express the Myb-regulated gene mim-1, suggesting that Myb requires tissue-specific, cooperating factors to activate such genes. Here, we investigated the tissue-specific regulation of the mim-1 promoter and found that it not only contains binding sites for Myb but also for NF-M, a myeloid-specific transcription factor that probably corresponds to mammalian C/EBP beta. Both types of binding sites were found to be required for full activity of the promoter. Remarkably, ectopic coexpression of Myb and NF-M proteins in erythroid cells or fibroblasts was sufficient to induce endogenous markers of myeloid differentiation, like the mim-1 and lysozyme genes. Our results indicate that c-Myb and NF-M proteins act as a bipartite, combinatorial signal that regulates the expression of myeloid-specific genes, even in heterologous cell types.
Keywords:Myb Oncogene, C/EBP-Like Factors, Hematopoiesis, Transcription Factors, Gene Expression, Animals, Chickens
Source:Genes & Development
ISSN:0890-9369
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Volume:7
Number:5
Page Range:749-759
Date:May 1993
Official Publication:https://doi.org/10.1101/gad.7.5.749
PubMed:View item in PubMed

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