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Kinase-controlled phase transition of membraneless organelles in mitosis

Item Type:Article
Title:Kinase-controlled phase transition of membraneless organelles in mitosis
Creators Name:Rai, A.K. and Chen, J.X. and Selbach, M. and Pelkmans, L.
Abstract:Liquid-liquid phase separation has been shown to underlie the formation and disassembly of membraneless organelles in cells, but the cellular mechanisms that control this phenomenon are poorly understood. A prominent example of regulated and reversible segregation of liquid phases may occur during mitosis, when membraneless organelles disappear upon nuclear-envelope breakdown and reappear as mitosis is completed. Here we show that the dual-specificity kinase DYRK3 acts as a central dissolvase of several types of membraneless organelle during mitosis. DYRK3 kinase activity is essential to prevent the unmixing of the mitotic cytoplasm into aberrant liquid-like hybrid organelles and the over-nucleation of spindle bodies. Our work supports a mechanism in which the dilution of phase-separating proteins during nuclear-envelope breakdown and the DYRK3-dependent degree of their solubility combine to allow cells to dissolve and condense several membraneless organelles during mitosis.
Source:Nature
ISSN:0028-0836
Publisher:Nature Publishing Group (U.K.)
Volume:559
Number:7713
Page Range:211-216
Date:July 2018
Official Publication:https://doi.org/10.1038/s41586-018-0279-8
PubMed:View item in PubMed

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