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Transient N-6-methyladenosine transcriptome sequencing reveals a regulatory role of m6A in splicing efficiency

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Item Type:Article
Title:Transient N-6-methyladenosine transcriptome sequencing reveals a regulatory role of m6A in splicing efficiency
Creators Name:Louloupi, A. and Ntini, E. and Conrad, T. and Ørom, U.A.V.
Abstract:Splicing efficiency varies among transcripts, and tight control of splicing kinetics is crucial for coordinated gene expression. N-6-methyladenosine (m6A) is the most abundant RNA modification and is involved in regulation of RNA biogenesis and function. The impact of m6A on regulation of RNA splicing kinetics is unknown. Here, we provide a time-resolved high-resolution assessment of m6A on nascent RNA transcripts and unveil its importance for the control of RNA splicing kinetics. We find that early co-transcriptional m6A deposition near splice junctions promotes fast splicing, while m6A modifications in introns are associated with long, slowly processed introns and alternative splicing events. In conclusion, we show that early m6A deposition specifies the fate of transcripts regarding splicing kinetics and alternative splicing.
Keywords:RNA Modifications, Nascent RNA, m6A, Splicing Dynamics, RNA, Alternative Splicing
Source:Cell Reports
ISSN:2211-1247
Publisher:Cell Press / Elsevier (U.S.A.)
Volume:23
Number:12
Page Range:3429-3437
Date:19 June 2018
Official Publication:https://doi.org/10.1016/j.celrep.2018.05.077
PubMed:View item in PubMed

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