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The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse

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Item Type:Article
Title:The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse
Creators Name:Bégay, V. and Baumeier, C. and Zimmermann, K. and Heuser, A. and Leutz, A.
Abstract:The transcription factor C/EBPβ regulates hematopoiesis, bone, liver, fat, and skin homeostasis, and female reproduction. C/EBPβ protein expression from its single transcript occurs by alternative in-frame translation initiation at consecutive start sites to generate three isoforms, two long (LAP*, LAP) and one truncated (LIP), with the same C-terminal bZip dimerization domain. The long C/EBPβ isoforms are considered gene activators, whereas the LIP isoform reportedly acts as a dominant-negative repressor. Here, we tested the putative repressor functions of the C/EBPβ LIP isoform in mice by comparing monoallelic WT or LIP knockin mice with Cebpb knockout mice, in combination with monoallelic Cebpa mice. The C/EBPβ LIP isoform was sufficient to function in coordination with C/EBPα in murine development, adipose tissue and sebocyte differentiation, and female fertility. Thus, the C/EBPβ LIP isoform likely has more physiological functions than its currently known role as a dominant-negative inhibitor, which are more complex than anticipated.
Keywords:3T3-L1 Cells, Adipose Tissue, Alleles, CCAAT-Enhancer-Binding Protein-beta, Fertility, Gene Knockout Techniques, Homeostasis, Phenotype, Protein Isoforms, Skin, Animals, Mice
Source:Scientific Reports
ISSN:2045-2322
Publisher:Nature Publishing Group (U.K.)
Volume:8
Number:1
Page Range:8417
Date:30 May 2018
Official Publication:https://doi.org/10.1038/s41598-018-26579-y
PubMed:View item in PubMed

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