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Isolation of native plasma membrane H(+)-ATPase (Pma1p) in both the active and basal activation states

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Item Type:Article
Title:Isolation of native plasma membrane H(+)-ATPase (Pma1p) in both the active and basal activation states
Creators Name:Pedersen, J.T. and Kanashova, T. and Dittmar, G. and Palmgren, M.
Abstract:The yeast plasma membrane H(+)-ATPase Pma1p is a P-type ATPase that energizes the yeast plasma membrane. Pma1p exists in two activation states: an autoinhibited basal state and an activated state. Here we show that functional and stable Pma1p can be purified in native form and reconstituted in artificial liposomes without altering its activation state. Acetylated tubulin has previously been reported to maintain Pma1p in the basal state but, as this protein was absent from the purified preparations, it cannot be an essential component of the autoinhibitory mechanism. Purification of and reconstitution of native Pma1p in both activation states opens up for a direct comparison of the transport properties of these states, which allowed us to confirm that the basal state has a low coupling ratio between ATP hydrolysis and protons pumped, whereas the activated state has a high coupling ratio. The ability to prepare native Pma1p in both activation states will facilitate further structural and biochemical studies examining the mechanism by which plasma membrane H(+)-ATPases are autoinhibited.
Keywords:Acetylated Tubulin, Autoinhibitory Regulation, Pma1p, P-Type ATPase
Source:FEBS Open Bio
Page Range:774-783
Date:May 2018
Official Publication:https://doi.org/10.1002/2211-5463.12413
PubMed:View item in PubMed

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