Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Simultaneous lineage tracing and cell-type identification using CRISPR-Cas9-induced genetic scars

Item Type:Letter
Title:Simultaneous lineage tracing and cell-type identification using CRISPR-Cas9-induced genetic scars
Creators Name:Spanjaard, B. and Hu, B. and Mitic, N. and Olivares-Chauvet, P. and Janjuha, S. and Ninov, N. and Junker, J.P.
Abstract:A key goal of developmental biology is to understand how a single cell is transformed into a full-grown organism comprising many different cell types. Single-cell RNA-sequencing (scRNA-seq) is commonly used to identify cell types in a tissue or organ. However, organizing the resulting taxonomy of cell types into lineage trees to understand the developmental origin of cells remains challenging. Here we present LINNAEUS (lineage tracing by nuclease-activated editing of ubiquitous sequences)-a strategy for simultaneous lineage tracing and transcriptome profiling in thousands of single cells. By combining scRNA-seq with computational analysis of lineage barcodes, generated by genome editing of transgenic reporter genes, we reconstruct developmental lineage trees in zebrafish larvae, and in heart, liver, pancreas, and telencephalon of adult fish. LINNAEUS provides a systematic approach for tracing the origin of novel cell types, or known cell types under different conditions.
Source:Nature Biotechnology
ISSN:1087-0156
Publisher:Nature Publishing Group (U.S.A.)
Date:9 April 2018
Official Publication:https://doi.org/10.1038/nbt.4124
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library