Alamandine acts via MrgD to induce AMPK/NO activation against Ang II hypertrophy in cardiomyocytes

Item Type:	Article
Title:	Alamandine acts via MrgD to induce AMPK/NO activation against Ang II hypertrophy in cardiomyocytes
Creators Name:	de Jesus, I.C.G. and Scalzo, S. and Alves, F. and Marques, K. and Rocha-Resende, C. and Bader, M. and Santos, R.A.S. and Guatimosim, S.
Abstract:	The renin-angiotensin system (RAS) plays a pivotal role in the pathogenesis of cardiovascular diseases. New members of this system have been characterized and shown to have biologically relevant actions. Alamandine and its receptor MrgD are recently identified components of RAS. In the cardiovascular system alamandine actions included vasodilation, antihypertensive and anti-fibrosis effects. Currently, the actions of alamandine on cardiomyocytes are unknown. Here our goal was twofold: (1) to unravel the signaling molecules activated by the alamandine/MrgD axis in cardiomyocytes; (2) to evaluate the ability of this axis to prevent against Angiotensin II (Ang II)-induced hypertrophy. In cardiomyocytes from C57BL/6 mice, alamandine treatment induced an increase in nitric oxide (NO) production, which was blocked by D-Pro-Ang-(1-7), a MrgD antagonist. This NO rise correlated with increased phosphorylation of AMPK. Alamandine induced NO production was preserved in Masmyocytes, and lost in MrgDcells. Binding of fluorescent-labeled alamandine was observed in wild-type cells, but it was dramatically reduced in MrgDmyocytes. We also assessed the consequences of prolonged alamandine exposure to cultured neonatal rat cardiomyocytes (NRCMs) treated with Ang II. Treatment of NRCMs with alamandine prevented Ang II-induced hypertrophy. Moreover, antihypertrophic actions of alamandine were mediated via MrgD and NO, since they could be prevented by D-Pro-Ang-(1-7) or inhibitors of NO synthase or AMPK. β-alanine, a MrgD agonist, recapitulated alamandine's cardioprotective effects in cardiomyocytes. Our data show that alamandine via MrgD induces AMPK/NO signaling to counterregulate Ang II induced hypertrophy. These findings highlight the therapeutic potential of the alamandine/MrgD axis in the heart.
Keywords:	AMP-Activated Protein Kinase, Angiotensin-System, Hypertrophy, Nitric Oxide, Ventricular Myocytes, Animals, Mice, Rats
Source:	American Journal of Physiology Cell Physiology
ISSN:	0363-6143
Publisher:	American Physiological Society
Volume:	314
Number:	6
Page Range:	C702-C711
Date:	June 2018
Official Publication:	https://doi.org/10.1152/ajpcell.00153.2017
PubMed:	View item in PubMed

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