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Vinculin binding angle in podosomes revealed by high resolution microscopy.

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Item Type:Article
Title:Vinculin binding angle in podosomes revealed by high resolution microscopy.
Creators Name:Walde, M. and Monypenny, J. and Heintzmann, R. and Jones, G.E. and Cox, S.
Abstract:Podosomes are highly dynamic actin-rich adhesive structures formed predominantly by cells of the monocytic lineage, which degrade the extracellular matrix. They consist of a core of F-actin and actin-regulating proteins, surrounded by a ring of adhesion-associated proteins such as vinculin. We have characterised the structure of podosomes in macrophages, particularly the structure of the ring, using three super-resolution fluorescence microscopy techniques: stimulated emission depletion microscopy, structured illumination microscopy and localisation microscopy. Rather than being round, as previously assumed, we found the vinculin ring to be created from relatively straight strands of vinculin, resulting in a distinctly polygonal shape. The strands bind preferentially at angles between 116° and 135°. Furthermore, adjacent vinculin strands are observed nucleating at the corners of the podosomes, suggesting a mechanism for podosome growth.
Source:PLoS ONE
ISSN:1932-6203
Publisher:Public Library of Science (U.S.A.)
Volume:9
Number:2
Page Range:e88251
Date:11 February 2014
Official Publication:https://doi.org/10.1371/journal.pone.0088251
PubMed:View item in PubMed

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