Single-molecule fluorescence in situ hybridization (FISH) of circular RNA CDR1as

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Item Type:	Article
Title:	Single-molecule fluorescence in situ hybridization (FISH) of circular RNA CDR1as
Creators Name:	Kocks, C., Boltengagen, A., Piwecka, M., Rybak-Wolf, A. and Rajewsky, N.
Abstract:	Individual mRNA molecules can be imaged in fixed cells by hybridization with multiple, singly labeled oligonucleotide probes, followed by computational identification of fluorescent signals. This approach, called single-molecule RNA fluorescence in situ hybridization (smRNA FISH), allows subcellular localization and absolute quantification of RNA molecules in individual cells. Here, we describe a simple smRNA FISH protocol for two-color imaging of a circular RNA, CDR1as, simultaneously with an unrelated messenger RNA. The protocol can be adapted to circRNAs that coexist with overlapping, noncircular mRNA isoforms produced from the same genetic locus.
Keywords:	circRNA Visualization, circRNA Quantification, FISH, Single-Molecule Detection, In Situ Hybridization, Absolute Quantification Of Gene Expression
Source:	Methods in Molecular Biology
Series Name:	Methods in Molecular Biology
Title of Book:	Circular RNAs
ISSN:	1064-3745
ISBN:	978-1-4939-7561-7
Publisher:	Springer / Humana Press
Volume:	1724
Page Range:	77-96
Date:	2018
Official Publication:	https://doi.org/10.1007/978-1-4939-7562-4_7
PubMed:	View item in PubMed

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