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Adipose tissue ATGL modifies the cardiac lipidome in pressure-overload-induced left ventricular failure

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Item Type:Article
Title:Adipose tissue ATGL modifies the cardiac lipidome in pressure-overload-induced left ventricular failure
Creators Name:Salatzki, J. and Foryst-Ludwig, A. and Bentele, K. and Blumrich, A. and Smeir, E. and Ban, Z. and Brix, S. and Grune, J. and Beyhoff, N. and Klopfleisch, R. and Dunst, S. and Surma, M.A. and Klose, C. and Rothe, M. and Heinzel, F.R. and Krannich, A. and Kershaw, E.E. and Beule, D. and Schulze, P.C. and Marx, N. and Kintscher, U.
Abstract:Adipose tissue lipolysis occurs during the development of heart failure as a consequence of chronic adrenergic stimulation. However, the impact of enhanced adipose triacylglycerol hydrolysis mediated by adipose triglyceride lipase (ATGL) on cardiac function is unclear. To investigate the role of adipose tissue lipolysis during heart failure, we generated mice with tissue-specific deletion of ATGL (atATGL-KO). atATGL-KO mice were subjected to transverse aortic constriction (TAC) to induce pressure-mediated cardiac failure. The cardiac mouse lipidome and the human plasma lipidome from healthy controls (n = 10) and patients with systolic heart failure (HFrEF, n = 13) were analyzed by MS-based shotgun lipidomics. TAC-induced increases in left ventricular mass (LVM) and diastolic LV inner diameter were significantly attenuated in atATGL-KO mice compared to wild type (wt) -mice. More importantly, atATGL-KO mice were protected against TAC-induced systolic LV failure. Perturbation of lipolysis in the adipose tissue of atATGL-KO mice resulted in the prevention of the major cardiac lipidome changes observed after TAC in wt-mice. Profound changes occurred in the lipid class of phosphatidylethanolamines (PE) in which multiple PE-species were markedly induced in failing wt-hearts which was attenuated in atATGL-KO hearts. Moreover, selected heart failure-induced PE species in mouse hearts were also induced in plasma samples from patients with chronic heart failure. TAC-induced cardiac PE induction resulted in decreased PC/ PE-species ratios associated with increased apoptotic marker expression in failing wt-hearts, a process absent in atATGL-KO hearts. Perturbation of adipose tissue lipolysis by ATGL-deficiency ameliorated pressure-induced heart failure and the potentially deleterious cardiac lipidome changes that accompany this pathological process, namely the induction of specific PE species. Non-cardiac ATGL-mediated modulation of the cardiac lipidome may play an important role in the pathogenesis of chronic heart failure.
Keywords:Adipose Tissue, Cardiac Myocytes, Case-Control Studies, Cultured Cells, Heart Failure, Hypertension, Knockout Mice, Left Ventricular Dysfunction, Lipase, Lipid Metabolism, Metabolome, Myocardium, Ventricular Remodeling, Animals, Mice
Source:PLoS Genetics
ISSN:1553-7404
Publisher:Public Library of Science (U.S.A.)
Volume:14
Number:1
Page Range:e1007171
Date:10 January 2018
Official Publication:https://doi.org/10.1371/journal.pgen.1007171
PubMed:View item in PubMed

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