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| Item Type: | Article |
|---|---|
| Title: | Cell-accurate optical mapping across the entire developing heart |
| Creators Name: | Weber, M. and Scherf, N. and Meyer, A.M. and Panáková, D. and Kohl, P. and Huisken, J. |
| Abstract: | Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca(2+)-mapping of all cells in the entire electro-mechanically uncoupled heart during the looping stage of live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs. |
| Keywords: | Cardiac Conduction, Cardiology, Light Sheet Microscopy, Optical Mapping, Organogenesis, Biophysics, Developmental Biology, Image Analysis, Stem Cells, Structural Biology, Animals, Zebrafish |
| Source: | eLife |
| ISSN: | 2050-084X |
| Publisher: | eLife Sciences Publications |
| Volume: | 6 |
| Page Range: | e28307 |
| Date: | 29 December 2017 |
| Official Publication: | https://doi.org/10.7554/eLife.28307 |
| PubMed: | View item in PubMed |
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