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Metabolic labeling of newly synthesized RNA with 4sU to in parallel assess RNA transcription and decay

Item Type:Article
Title:Metabolic labeling of newly synthesized RNA with 4sU to in parallel assess RNA transcription and decay
Creators Name:Sun, W. and Chen, W.
Abstract:The development of genome-wide RNA profiling technologies greatly facilitates the global analysis of gene expression. However, such technologies alone could not distinguish the contribution to cellular RNA abundance by transcription versus decay. To overcome such limitation, metabolic labeling of newly synthesized RNA with 4-thiouridine (4sU) combined with genome-wide RNA profiling was used to in parallel measure RNA transcription and decay. Here, we describe the detailed protocol for using metabolic labeling with 4sU to separate newly synthesized RNA from the preexisting RNA in mammalian cells.
Keywords:4-Thiouridine(4sU), Metabolic Labeling, Newly Synthesized RNA, RNA Transcription, RNA Decay, Global Analysis
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:mRNA decay: methods and Protocols
ISSN:1064-3745
ISBN:978-1-4939-7539-6
Publisher:Springer / Humana Press (U.S.A.)
Volume:1720
Page Range:25-34
Number of Pages:10
Date:2018
Official Publication:https://doi.org/10.1007/978-1-4939-7540-2_3
PubMed:View item in PubMed

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