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Transcriptome-wide identification of protein binding sites on RNA by PAR-CLIP (photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation)

Item Type:Book Section
Title:Transcriptome-wide identification of protein binding sites on RNA by PAR-CLIP (photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation)
Creators Name:Hoell, J.I. and Hafner, M. and Landthaler, M. and Ascano, M. and Farazi, T.A. and Wardle, G. and Nusbaum, J. and Cekan, P. and Khorshid, M. and Burger, L. and Zavolan, M. and Tuschl, T.
Keywords:Immunoprecipitation, Crosslinking, 4-Thiouridine (4SU), 6-Thioguanosine (6SG), Preadenylated Adapter, RNA Size Markers, cDNA Library Preparation, Radiolabeling of RNA Size Markers, RNA Binding Protein (RBP), UV-Croslinking of 4SU-Labeled Cells, Magnetic Beads for Immunoprecipitation, RNase T1, SDS-PAGE, Electroelution of RNA-RBP Complexes, Proteinase K, Adapter Ligation, Reverse Transcription, PCR, Illumina Sequencing of cDNAs, Reversed Phase HPLC (RP-HPLC), Determination of 4SU Incorporation Levels
Source:Handbook of RNA Biochemistry: Second, Completely Revised and Enlarged Edition
Title of Book:Handbook of RNA Biochemistry: Second, Completely Revised and Enlarged Edition
ISBN:9783527327645
Publisher:Wiley-VCH (Germany)
Volume:2
Page Range:877-898
Date:2014
Official Publication:https://doi.org/10.1002/9783527647064.ch39

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