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Dynamic profiling of the protein life cycle in response to pathogens

Item Type:Article
Title:Dynamic profiling of the protein life cycle in response to pathogens
Creators Name:Jovanovic, M. and Rooney, M.S. and Mertins, P. and Przybylski, D. and Chevrier, N. and Satija, R. and Rodriguez, E.H. and Fields, A.P. and Schwartz, S. and Raychowdhury, R. and Mumbach, M.R. and Eisenhaure, T. and Rabani, M. and Gennert, D. and Lu, D. and Delorey, T. and Weissman, J.S. and Carr, S.A. and Hacohen, N. and Regev, A.
Abstract:Protein expression is regulated by the production and degradation of messenger RNAs (mRNAs) and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics so as to build a quantitative genomic model of the differential regulation of gene expression in lipopolysaccharide-stimulated mouse dendritic cells. Changes in mRNA abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for more than half of the absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction for newly activated cellular functions and by protein life-cycle changes for remodeling of preexisting functions.
Keywords:Amino Acids, Bone Marrow Cells, Cell Culture Techniques, Dendritic Cells, Host-Pathogen Interactions, Isotope Labeling, Lipopolysaccharides, Messenger RNA, Mitochondrial Proteins, Molecular Dynamics Simulation, Protein Biosynthesis, Proteolysis, RNA Sequence Analysis, Animals, Mice
Publisher:American Association for the Advancement of Science
Page Range:1259038
Date:6 March 2015
Official Publication:https://doi.org/10.1126/science.1259038
PubMed:View item in PubMed

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