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Integrated proteomic analysis of post-translational modifications by serial enrichment

Item Type:Article
Title:Integrated proteomic analysis of post-translational modifications by serial enrichment
Creators Name:Mertins, P. and Qiao, J.W. and Patel, J. and Udeshi, N.D. and Clauser, K.R. and Mani, D.R. and Burgess, M.W. and Gillette, M.A. and Jaffe, J.D. and Carr, S.A.
Abstract:We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.
Keywords:Gene Expression Profiling, Mass Spectrometry, Post-Translational Protein Processing, Proteome, Systems Integration
Source:Nature Methods
ISSN:1548-7091
Publisher:Nature Publishing Group (U.S.A.)
Volume:10
Number:7
Page Range:634-637
Date:July 2013
Official Publication:https://doi.org/10.1038/nmeth.2518
PubMed:View item in PubMed

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