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A map of human circular RNAs in clinically relevant tissues

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Item Type:Article
Title:A map of human circular RNAs in clinically relevant tissues
Creators Name:Maass, P.G. and Glažar, P. and Memczak, S. and Dittmar, G. and Hollfinger, I. and Schreyer, L. and Sauer, A.V. and Toka, O. and Aiuti, A. and Luft, F.C. and Rajewsky, N.
Abstract:Cellular circular RNAs (circRNAs) are generated by head-to-tail splicing and are present in all multicellular organisms studied so far. Recently, circRNAs have emerged as a large class of RNA which can function as post-transcriptional regulators. It has also been shown that many circRNAs are tissue- and stage-specifically expressed. Moreover, the unusual stability and expression specificity make circRNAs important candidates for clinical biomarker research. Here, we present a circRNA expression resource of 20 human tissues highly relevant to disease-related research: vascular smooth muscle cells (VSMCs), human umbilical vein cells (HUVECs), artery endothelial cells (HUAECs), atrium, vena cava, neutrophils, platelets, cerebral cortex, placenta, and samples from mesenchymal stem cell differentiation. In eight different samples from a single donor, we found highly tissue-specific circRNA expression. Circular-to-linear RNA ratios revealed that many circRNAs were expressed higher than their linear host transcripts. Among the 71 validated circRNAs, we noticed potential biomarkers. In adenosine deaminase-deficient, severe combined immunodeficiency (ADA-SCID) patients and in Wiskott-Aldrich-Syndrome (WAS) patients' samples, we found evidence for differential circRNA expression of genes that are involved in the molecular pathogenesis of both phenotypes. Our findings underscore the need to assess circRNAs in mechanisms of human disease.
Keywords:Circular RNAs, circRNA Catalog, Potential Biomarker, Human Cell Types
Source:Journal of Molecular Medicine
Page Range:1179-1189
Date:November 2017
Official Publication:https://doi.org/10.1007/s00109-017-1582-9
PubMed:View item in PubMed

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