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Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition

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Item Type:Article
Title:Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition
Creators Name:Alberti, A. and Poulain, J. and Engelen, S. and Labadie, K. and Romac, S. and Ferrera, I. and Albini, G. and Aury, J.M. and Belser, C. and Bertrand, A. and Cruaud, C. and Da Silva, C. and Dossat, C. and Gavory, F. and Gas, S. and Guy, J. and Haquelle, M. and Jacoby, E. and Jaillon, O. and Lemainque, A. and Pelletier, E. and Samson, G. and Wessner, M. and Acinas, S.G. and Royo-Llonch, M. and Cornejo-Castillo, F.M. and Logares, R. and Fernandez-Gomez, B. and Bowler, C. and Cochrane, G. and Amid, C. and Hoopen, P.T. and De Vargas, C. and Grimsley, N. and Desgranges, E. and Kandels-Lewis, S. and Ogata, H. and Poulton, N. and Sieracki, M.E. and Stepanauskas, R. and Sullivan, M.B. and Brum, J.R. and Duhaime, M.B. and Poulos, B.T. and Hurwitz, B.L. and Pesant, S. and Karsenti, E. and Wincker, P.
Abstract:A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world's planktonic ecosystems.
Keywords:Ecosystem, Genomics, Nucleotides, Oceans and Seas, Plankton, Viruses
Source:Scientific Data
Page Range:170093
Date:1 August 2017
Additional Information:Peer Bork is a coordinator of the Tara Oceans Consortium.
Official Publication:https://doi.org/10.1038/sdata.2017.93
PubMed:View item in PubMed

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