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Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function

Official URL:https://doi.org/10.1126/science.aam8526
PubMed:View item in PubMed
Creators Name:Piwecka, M. and Glazar, Petar and Hernandez-Miranda, L.R. and Memczak, S. and Wolf, S.A. and Rybak-Wolf, A. and Filipchyk, A. and Klironomos, F. and Cerda Jara, C.A. and Fenske, P. and Trimbuch, T. and Zywitza, V. and Plass, M. and Schreyer, L. and Ayoub, S. and Kocks, C. and Kuehn, R. and Rosenmund, C. and Birchmeier, C. and Rajewsky, N.
Journal Title:Science
Journal Abbreviation:Science
Date:10 August 2017
Abstract:Hundreds of circular RNAs (circRNAs) are highly abundant in mammalian brain, with oftentimes conserved expression. Here, we show that the circRNA Cdr1as is massively bound by miR-7 and miR-671 in the human and mouse brain. When the Cdr1as locus was removed from the mouse genome, knockout animals displayed impaired sensorimotor gating, a deficit in the ability to filter out unnecessary information associated with neuropsychiatric disorders. Electrophysiological recordings revealed dysfunctional synaptic transmission. Expression of microRNAs miR-7 and miR-671 was specifically and post-transcriptionally misregulated in all brain regions analyzed. Expression of immediate early genes such as Fos, a direct miR-7 target, was enhanced in Cdr1as-deficient brains, providing a possible molecular link to the behavioral phenotype. Our data indicate an in vivo loss-of-function circRNA phenotype and suggest that interactions between circRNAs and miRNAs are important for normal brain function.
ISSN:0036-8075
Publisher:American Association for the Advancement of Science (U.S.A.)
Item Type:Article

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