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Synapsin-antibodies in psychiatric and neurological disorders: prevalence and clinical findings

Item Type:Article
Title:Synapsin-antibodies in psychiatric and neurological disorders: prevalence and clinical findings
Creators Name:Höltje, M. and Mertens, R. and Schou, M.B. and Saether, S.G. and Kochova, E. and Jarius, S. and Prüss, H. and Komorowski, L. and Probst, C. and Paul, F. and Bellmann-Strobl, J. and Gitler, D. and Benfenati, F. and Piepgras, J. and Ahnert-Hilger, G. and Ruprecht, K.
Abstract:OBJECTIVE: To study the prevalence of autoantibodies to synapsin in patients with psychiatric and neurological disorders and to describe clinical findings in synapsin antibody positive patients. METHODS: Sera of 375 patients with different psychiatric and neurological disorders and sera of 97 healthy controls were screened (dilution 1:320) for anti-synapsin IgG using HEK293 cells transfected with rat synapsin Ia. Positive sera were further analyzed by immunoblots with brain tissue from wild type and synapsin knock out mice and with HEK293 cells transfected with human synapsin Ia and Ib. Binding of synapsin IgG positive sera to primary neurons was studied using murine hippocampal neurons. RESULTS: IgG in serum from 23 (6.1%) of 375 patients, but from none of the 97 healthy controls (p=0.007), bound to rat synapsin Ia transfected cells with a median (range) titer of 1:1000 (1:320 -1:100,000). Twelve of the 23 positive sera reacted with a protein of the molecular size of synapsin I in immunoblots of wild type but not of synapsin knock out mouse brain tissue. Out of 19/23 positive sera available for testing, 13 bound to human synapsin Ia and 16 to human synapsin 1b transfected cells. Synapsin IgG positive sera stained fixed and permeabilized murine hippocampal neurons. Synapsin IgG positive patients had various psychiatric and neurological disorders. Tumors were documented in 2 patients (melanoma, small cell lung carcinoma); concomitant anti-neuronal or other autoantibodies were present in 8 patients. CONCLUSIONS: Autoantibodies to human synapsin Ia and Ib are detectable in a proportion of sera from patients with different psychiatric and neurological disorders, warranting further investigation into the potential pathophysiological relevance of these antibodies.
Keywords:Synapsin, Antibodies, Psychiatric Disorders, Neurologic Disorders, Demyelinating Disease, Autoimmunity, Serum, Animals, Mice, Rats
Source:Brain Behavior and Immunity
Publisher:Elsevier / Academic Press
Page Range:125-134
Date:November 2017
Official Publication:https://doi.org/10.1016/j.bbi.2017.07.011
PubMed:View item in PubMed

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