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| Item Type: | Review |
|---|---|
| Title: | Tissue specific labeling in proteomics |
| Creators Name: | Ramberger, E. and Dittmar, G. |
| Abstract: | Mass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in multicellular organisms and tissues poses a significant challenge. Isolating a subset of cells from tissues requires mechanical and biochemical separation or sorting, a process which can alter cellular signaling, and thus, the composition of the proteome. Recently, several approaches for cell selective labeling of proteins, that include bioorthogonal amino acids, biotinylating enzymes, and genetic tools, have been developed. These tools facilitate the selective labeling of proteins, their interactome, or of specific cell types within a tissue or an organism, while avoiding the difficult and contamination-prone biochemical separation of cells from the tissue. In this review, we give an overview of existing techniques and their application in cell culture models and whole animals. |
| Keywords: | Quantitative Proteomics, Stable Isotope Labeling, Tissue, Amino Acid Analog, Biotinylation, Bioorthogonal, Multicellular, SILAC, APEX, BioID, Animals |
| Source: | Proteomes |
| ISSN: | 2227-7382 |
| Publisher: | MDPI |
| Volume: | 5 |
| Number: | 3 |
| Page Range: | E17 |
| Date: | 18 July 2017 |
| Official Publication: | https://doi.org/10.3390/proteomes5030017 |
| PubMed: | View item in PubMed |
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