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Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets

Official URL:https://doi.org/10.1084/jem.20161093
PubMed:View item in PubMed
Creators Name:Eulenberg-Gustavus, C. and Baehring, S. and Maass, P. and Luft, F.C. and Kettritz, R.
Journal Title:Journal of Experimental Medicine
Journal Abbreviation:J Exp Med
Page Range:2089-2101
Date:3 July 2017
Abstract:CD177 presents antigens in allo- and autoimmune diseases on the neutrophil surface. Individuals can be either CD177-deficient or harbor distinct CD177(neg) and CD177(pos) neutrophil subsets. We studied mechanisms controlling subset-restricted CD177 expression in bimodal individuals. CD177(pos), but not CD177(neg) neutrophils, produced CD177 protein and mRNA. Haplotype analysis indicated a unique monoallelic CD177 expression pattern, where the offspring stably transcribed either the maternal or paternal allele. Hematopoietic stem cells expressed both CD177 alleles and silenced one copy during neutrophil differentiation. ChIP and reporter assays in HeLa cells with monoallelic CD177 expression showed that methylation reduced reporter activity, whereas demethylation caused biallelic CD177 expression. HeLa cell transfection with c-Jun and c-Fos increased CD177 mRNA. Importantly, CD177(pos) human neutrophils, but not CD177(neg) neutrophils, showed a euchromatic CD177 promoter, unmethylated CpGs, and c-Jun and c-Fos binding. We describe epigenetic mechanisms explaining the two distinct CD177 neutrophil subsets and a novel monoallelic CD177 expression pattern that does not follow classical random monoallelic expression or imprinting.
Publisher:Rockefeller University Press (U.S.A.)
Item Type:Article

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