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Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets

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Item Type:Article
Title:Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets
Creators Name:Eulenberg-Gustavus, C. and Bähring, S. and Maass, P. and Luft, F.C. and Kettritz, R.
Abstract:CD177 presents antigens in allo- and autoimmune diseases on the neutrophil surface. Individuals can be either CD177-deficient or harbor distinct CD177(neg) and CD177(pos) neutrophil subsets. We studied mechanisms controlling subset-restricted CD177 expression in bimodal individuals. CD177(pos), but not CD177(neg) neutrophils, produced CD177 protein and mRNA. Haplotype analysis indicated a unique monoallelic CD177 expression pattern, where the offspring stably transcribed either the maternal or paternal allele. Hematopoietic stem cells expressed both CD177 alleles and silenced one copy during neutrophil differentiation. ChIP and reporter assays in HeLa cells with monoallelic CD177 expression showed that methylation reduced reporter activity, whereas demethylation caused biallelic CD177 expression. HeLa cell transfection with c-Jun and c-Fos increased CD177 mRNA. Importantly, CD177(pos) human neutrophils, but not CD177(neg) neutrophils, showed a euchromatic CD177 promoter, unmethylated CpGs, and c-Jun and c-Fos binding. We describe epigenetic mechanisms explaining the two distinct CD177 neutrophil subsets and a novel monoallelic CD177 expression pattern that does not follow classical random monoallelic expression or imprinting.
Keywords:Alleles, Base Sequence, Cell Differentiation, CpG Islands, DNA Methylation, Fetal Blood, GPI-Linked Proteins, Gene Expression Profiling, Gene Silencing, HeLa Cells, Hematopoietic Stem Cells, Immunoblotting, Isoantigens, Neutrophils, Promoter Regions, Genetic, Receptors, Cell Surface, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Transcription Factor AP-1
Source:Journal of Experimental Medicine
Publisher:Rockefeller University Press
Page Range:2089-2101
Date:3 July 2017
Official Publication:https://doi.org/10.1084/jem.20161093
PubMed:View item in PubMed

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