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Control of gene editing by manipulation of DNA repair mechanisms

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Item Type:Article
Title:Control of gene editing by manipulation of DNA repair mechanisms
Creators Name:Danner, E. and Bashir, S. and Yumlu, S. and Wurst, W. and Wefers, B. and Kühn, R.
Abstract:DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to achieve genome editing through DSB repair. These breaks are repaired by one of two main repair pathways, classic non-homologous end joining (c-NHEJ) and homology-directed repair (HDR), the latter being restricted to the S/G2 phases of the cell cycle and notably less frequent. Precise genome editing applications rely on HDR, with the abundant c-NHEJ formed mutations presenting a barrier to achieving high rates of precise sequence modifications. Here, we give an overview of HDR- and c-NHEJ-mediated DSB repair in gene editing and summarize the current efforts to promote HDR over c-NHEJ.
Keywords:Biomarkers, CRISPR-Cas Systems, DNA End-Joining Repair, DNA Repair, Gene Editing, Gene Knock-In Techniques, Gene Knockout Techniques, Genetic Testing, Homologous Recombination, Recombinational DNA Repair, Signal Transduction, Animals
Source:Mammalian Genome
ISSN:0938-8990
Publisher:Springer (U.S.A.)
Volume:28
Number:7-8
Page Range:262-274
Date:August 2017
Additional Information:Copyright © 2017 Springer Science+Business Media New York
Official Publication:https://doi.org/10.1007/s00335-017-9688-5
PubMed:View item in PubMed

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