Helmholtz Gemeinschaft


Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression

PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Item Type:Preprint
Title:Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression
Creators Name:Kar, G. and Kim, J.K. and Kolodziejczyk, A.A. and Natarajan, K.N. and Torlai Triglia, E. and Mifsud, B. and Elderkin, S. and Marioni, J.C. and Pombo, A. and Teichmann, S.A.
Abstract:Polycomb repressive complexes (PRCs) are important histone modifiers, which silence gene expression, yet there exists a subset of PRC-bound genes actively transcribed by RNA polymerase II (RNAPII). It is likely that the role of PRC is to dampen expression of these PRC-active genes. However, it is unclear how this flipping between chromatin states alters the kinetics of transcriptional burst size and frequency relative to genes with exclusively activating marks. To investigate this, we integrate histone modifications and RNAPII states derived from bulk ChIP-seq data with single-cell RNA-sequencing data. We find that PRC-active genes have a greater cell-to-cell variation in expression than active genes with the same mean expression levels, and validate these results by knockout experiments. We also show that PRCactive genes are clustered on chromosomes in both two and three dimensions, and interactions with active enhancers promote a stabilization of gene expression noise. These findings provide new insights into how chromatin regulation modulates stochastic gene expression and transcriptional bursting, with implications for regulation of pluripotency and development.
Publisher:Cold Spring Harbor Laboratory Press
Article Number:117267
Date:16 March 2017
Official Publication:https://doi.org/10.1101/117267
Related to:
https://edoc.mdc-berlin.de/16723/Final version

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library