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Transposition of the bamboo Mariner-like element Ppmar1 in yeast

Item Type:Article
Title:Transposition of the bamboo Mariner-like element Ppmar1 in yeast
Creators Name:Zhou, M.B. and Hu, H. and Miskey, C. and Lazarow, K. and Ivics, Z. and Kunze, R. and Yang, G. and Izsvák, Z. and Tang, D.Q.
Abstract:The moso bamboo genome contains the two structurally intact and thus potentially functional mariner-like elements Ppmar1 and Ppmar2. Both elements contain perfect terminal inverted repeats (TIRs) and a full-length intact transposase gene. Here we investigated whether Ppmar1 is functional in yeast (Saccharomyces cerevisiae). We have designed a two-component system consisting of a transposase expression cassette and a non-autonomous transposon on two separate plasmids. We demonstrate that the Ppmar1 transposase Pptpase1 catalyses excision of the non-autonomous Ppmar1NA element from the plasmid and reintegration at TA dinucleotide sequences in the yeast chromosomes. In addition we generated 14 hyperactive Ppmar1 transposase variants by systematic single amino acid substitutions. The most active transposase variant, S171A, induces 10-fold more frequent Ppmar1NA excisions in yeast than the wild type transposase. The Ppmar1 transposon is a promising tool for insertion mutagenesis in moso bamboo and may be used in other plants as an alternative to the established transposon tagging systems.
Keywords:Mariner-Like Element, Transposase, Mutagenesis, Moso Bamboo (Phyllostachys edulis (Carriere) J. Houz.)
Source:Molecular Phylogenetics and Evolution
Publisher:Elsevier / Academic Press
Page Range:367-374
Date:April 2017
Official Publication:https://doi.org/10.1016/j.ympev.2017.02.005
PubMed:View item in PubMed

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