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EBI2 is highly expressed in multiple sclerosis lesions and promotes early CNS migration of encephalitogenic CD4 T cells

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Item Type:Article
Title:EBI2 is highly expressed in multiple sclerosis lesions and promotes early CNS migration of encephalitogenic CD4 T cells
Creators Name:Wanke, F. and Moos, S. and Croxford, A.L. and Heinen, A.P. and Gräf, S. and Kalt, B. and Tischner, D. and Zhang, J. and Christen, I. and Bruttger, J. and Yogev, N. and Tang, Y. and Zayoud, M. and Israel, N. and Karram, K. and Reißig, S. and Lacher, S.M. and Reichhold, C. and Mufazalov, I.A. and Ben-Nun, A. and Kuhlmann, T. and Wettschureck, N. and Sailer, A.W. and Rajewsky, K. and Casola, S. and Waisman, A. and Kurschus, F.C.
Abstract:Arrival of encephalitogenic T cells at inflammatory foci represents a critical step in development of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. EBI2 and its ligand, 7{alpha},25-OHC, direct immune cell localization in secondary lymphoid organs. CH25H and CYP7B1 hydroxylate cholesterol to 7{alpha},25-OHC. During EAE, we found increased expression of CH25H by microglia and CYP7B1 by CNS-infiltrating immune cells elevating the ligand concentration in the CNS. Two critical pro-inflammatory cytokines, interleukin-23 (IL-23) and interleukin-1 beta (IL-1{beta}), maintained expression of EBI2 in differentiating Th17 cells. In line with this, EBI2 enhanced early migration of encephalitogenic T cells into the CNS in a transfer EAE model. Nonetheless, EBI2 was dispensable in active EAE. Human Th17 cells do also express EBI2, and EBI2 expressing cells are abundant within multiple sclerosis (MS) white matter lesions. These findings implicate EBI2 as a mediator of CNS autoimmunity and describe mechanistically its contribution to the migration of autoreactive T cells into inflamed organs.
Keywords:Autoimmunity, CD4-Positive T-Lymphocytes, Cell Movement, Central Nervous System, Cytochrome P450 Family 7, Disease Models, Animal, Encephalomyelitis, Autoimmune, Experimental, Interleukin-1beta, Interleukin-23, Multiple Sclerosis, Receptors, G-Protein-Coupled, Steroid Hydroxylases, Th17 Cells, Animals, Mice, Mice, Inbred C57BL
Source:Cell Reports
ISSN:2211-1247
Publisher:Cell Press / Elsevier (U.S.A.)
Volume:18
Number:5
Page Range:1270-1284
Date:31 January 2017
Official Publication:https://doi.org/10.1016/j.celrep.2017.01.020
PubMed:View item in PubMed

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