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C/EBP-induced transdifferentiation reveals granulocyte-macrophage precursor-like plasticity of B cells

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Item Type:Article
Title:C/EBP-induced transdifferentiation reveals granulocyte-macrophage precursor-like plasticity of B cells
Creators Name:Cirovic, B. and Schönheit, J. and Kowenz-Leutz, E. and Ivanovska, J. and Klement, C. and Pronina, N. and Bégay, V. and Leutz, A.
Abstract:The lymphoid-myeloid transdifferentiation potentials of members of the C/EBP family (C/EBP{alpha}, {beta}, {delta}, and {epsilon}) were compared in v-Abl-immortalized primary B cells. Conversion of B cells to macrophages was readily induced by the ectopic expression of any C/EBP, and enhanced by endogenous C/EBP{alpha} and {beta} activation. High transgene expression of C/EBP{beta} or C/EBP{epsilon}, but not of C/EBP{alpha} or C/EBP{delta}, also induced the formation of granulocytes. Granulocytes and macrophages emerged in a mutually exclusive manner. C/EBP{beta}-expressing B cells produced granulocyte-macrophage progenitor (GMP)-like progenitors when subjected to selective pressure to eliminate lymphoid cells. The GMP-like progenitors remained self-renewing and cytokine-independent, and continuously produced macrophages and granulocytes. In addition to their suitability to study myelomonocytic lineage bifurcation, lineage-switched GMP-like progenitors could reflect the features of the lympho-myeloid lineage switch observed in leukemic progression.
Keywords:Hematopoiesis, Leukemia, Differentiation, Stem Cell, Progenitor, Lineage Switch, Animals, Mice
Source:Stem Cell Reports
Publisher:Cell Press / Elsevier
Page Range:346-359
Date:14 February 2017
Official Publication:https://doi.org/10.1016/j.stemcr.2016.12.015
PubMed:View item in PubMed

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