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Bacterial toxins for oncoleaking suicidal cancer gene therapy

Item Type:Article
Title:Bacterial toxins for oncoleaking suicidal cancer gene therapy
Creators Name:Pahle, J. and Walther, W.
Abstract:For suicide gene therapy, initially prodrug-converting enzymes (gene-directed enzyme-producing therapy, GDEPT) were employed to intracellularly metabolize non-toxic prodrugs into toxic compounds, leading to the effective suicidal killing of the transfected tumor cells. In this regard, the suicide gene therapy has demonstrated its potential for efficient tumor eradication. Numerous suicide genes of viral or bacterial origin were isolated, characterized, and extensively tested in vitro and in vivo, demonstrating their therapeutic potential even in clinical trials to treat cancers of different entities. Apart from this, growing efforts are made to generate more targeted and more effective suicide gene systems for cancer gene therapy. In this regard, bacterial toxins are an alternative to the classical GDEPT strategy, which add to the broad spectrum of different suicide approaches. In this context, lytic bacterial toxins, such as streptolysin O (SLO) or the claudin-targeted Clostridium perfringens enterotoxin (CPE) represent attractive new types of suicide oncoleaking genes. They permit as pore-forming proteins rapid and also selective toxicity toward a broad range of cancers. In this chapter, we describe the generation and use of SLO as well as of CPE-based gene therapies for the effective tumor cell eradication as promising, novel suicide gene approach particularly for treatment of therapy refractory tumors.
Keywords:Cancer Gene Therapy, Suicide Gene Therapy, Bacterial Toxin, Solid Tumors
Source:Recent Results in Cancer Research
Series Name:Recent Results in Cancer Research
Title of Book:Current Strategies in Cancer Gene Therapy
Publisher:Springer International Publishing (Switzerland)
Page Range:95-110
Number of Pages:121
Official Publication:https://doi.org/10.1007/978-3-319-42934-2_7
PubMed:View item in PubMed

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