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| Item Type: | Article |
|---|---|
| Title: | Glucagon-producing cells are increased in Mas-deficient mice |
| Creators Name: | Felix Braga, J. and Ravizzoni Dartora, D. and Alenina, N. and Bader, M. and Santos, R.A.S. |
| Abstract: | It has been shown that Angiotensin-(1-7) [Ang-(1-7)] produces several effects related to glucose homeostasis. In this study, we aimed to investigate the effects of genetic deletion of Ang-(1-7), the GPCR Mas, on the glucagon -producing cells. C57BL6/N Mas-/- mice presented a significant and marked increase in pancreatic {alpha}-cells (number of cells: 146 +/- 21 vs. 67 +/- 8 in WT - p<0.001) and the percentage per islet (17.9±0.91 vs. 12.3 +/- 0.9% in WT - p<0.0001) with subsequent reduction of {beta}-cells percentage (82.1 +/- 0.91 vs. 87.7 +/- 0.9% in WT - p<0.0001). Accordingly, glucagon plasma levels were increased (516.7 +/- 36.35 vs 390.8 +/- 56.45 pg/mL in WT, p<0.05 ) and insulin plasma levels were decreased in C57BL6/N Mas-/- mice (0.25 +/- 0.01 vs 0.31 +/- 56.45 pg/mL in WT- p=0.02). In order to eliminate the possibility of a background-related phenotype we determined the number of glucagon-producing cells in FVB/N Mas-/- mice. In keeping with the observations in C57BL6/N Mas-/- mice, the number and percentage of pancreatic {alpha}-cells were also significantly increased in these mice (number of {alpha}-cells: 260 +/- 22 vs. 156 +/- 12 in WT, p<0.001; percentage per islet: 16 +/- 0.8 vs. 10 +/- 0.5% in WT, p<0.0001). These results suggest that Mas has a previously unexpected role on the pancreatic glucagon production. |
| Keywords: | Metabolsim, Diabetes, Animals, Mice |
| Source: | Endocrine Connections |
| ISSN: | 2049-3614 |
| Publisher: | BioScientifica |
| Volume: | 6 |
| Number: | 1 |
| Page Range: | 27-32 |
| Date: | January 2017 |
| Official Publication: | https://doi.org/10.1530/EC-16-0098 |
| PubMed: | View item in PubMed |
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