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Epigenetics and control of RNAs

Item Type:Article
Title:Epigenetics and control of RNAs
Creators Name:Maatz, H. and van Heesch, S. and Kreuchwig, F. and Faber, A. and Adami, E. and Hubner, N. and Heinig, M.
Abstract:Histone modifications are epigenetic marks that fundamentally impact the regulation of gene expression. Integrating histone modification information in the analysis of gene expression traits (eQTL mapping) has been shown to significantly enhance the prediction of eQTLs. In this chapter, we describe (1) how to perform quantitative trait locus (QTL) analysis using histone modification levels as traits and (2) how to integrate these data with information on RNA expression for the elucidation of the epigenetic control of transcript levels. We will provide a comprehensive introduction into the topic, describe in detail how ChIP-seq data are analyzed and elaborate on how to integrate ChIP-seq and RNA-seq data from a segregating disease animal model for the identification of the epigenetic control of RNA expression.
Keywords:Histone Modifications, RNA Expression, ChIP-Seq, Integrative Analysis, Recombinant Inbred Panel, QTL Mapping
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:System genetics : methods and protocols
Publisher:Springer / Humana Press
Page Range:217-237
Number of Pages:609
Official Publication:https://doi.org/10.1007/978-1-4939-6427-7_9
PubMed:View item in PubMed

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