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Alternative splicing substantially diversifies the transcriptome during early photomorphogenesis and correlates with the energy availability in arabidopsis

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Item Type:Article
Title:Alternative splicing substantially diversifies the transcriptome during early photomorphogenesis and correlates with the energy availability in arabidopsis
Creators Name:Hartmann, L. and Drewe-Boss, P. and Wiessner, T. and Wagner, G. and Geue, S. and Lee, H.C. and Obermueller, D.M. and Kahles, A. and Behr, J. and Sinz, F.H. and Raetsch, G. and Wachter, A.
Abstract:Plants use light as source of energy and information to detect diurnal rhythms and seasonal changes. Sensing changing light conditions is critical to adjust plant metabolism and to initiate developmental transitions. Here we analyzed transcriptome-wide alterations in gene expression and alternative splicing (AS) of etiolated seedlings undergoing photomorphogenesis upon exposure to blue, red, or white light. Our analysis revealed massive transcriptome reprograming as reflected by differential expression of ~20% of all genes and changes in several hundred AS events. For more than 60% of all regulated AS events, light promoted the production of a presumably protein-coding variant at the expense of an mRNA with nonsense-mediated decay-triggering features. Accordingly, AS of the putative splicing factor REDUCED RED-LIGHT RESPONSES IN CRY1CRY2 BACKGROUND 1 (RRC1), previously identified as a red light signaling component, was shifted to the functional variant under light. Downstream analyses of candidate AS events pointed at a role of photoreceptor signaling only in monochromatic but not in white light. Furthermore, we demonstrated similar AS changes upon light exposure and exogenous sugar supply, with a critical involvement of kinase signaling. We propose that AS is an integration point of signaling pathways that sense and transmit information regarding the energy availability in plants.
Keywords:Alternative Splicing, Arabidopsis, Arabidopsis Proteins, Plant Gene Expression Regulation, Signal Transduction, Transcriptome
Source:Plant Cell
ISSN:1040-4651
Publisher:American Society of Plant Biologists (U.S.A.)
Volume:28
Number:11
Page Range:2715-2734
Date:November 2016
Official Publication:https://doi.org/10.1105/tpc.16.00508
PubMed:View item in PubMed

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