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The ITS2 Database III-sequences and structures for phylogeny

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Item Type:Article
Title:The ITS2 Database III-sequences and structures for phylogeny
Creators Name:Koetschan, C. and Foerster, F. and Keller, A. and Schleicher, T. and Ruderisch, B. and Schwarz, R. and Mueller, T. and Wolf, M. and Schultz, J.
Abstract:The internal transcribed spacer 2 (ITS2) is a widely used phylogenetic marker. In the past, it has mainly been used for species level classifications. Nowadays, a wider applicability becomes apparent. Here, the conserved structure of the RNA molecule plays a vital role. We have developed the ITS2 Database (http://its2.bioapps.biozentrum.uni-wuerzburg.de) which holds information about sequence, structure and taxonomic classification of all ITS2 in GenBank. In the new version, we use Hidden Markov models (HMMs) for the identification and delineation of the ITS2 resulting in a major redesign of the annotation pipeline. This allowed the identification of more than 160,000 correct full length and more than 50,000 partial structures. In the web interface, these can now be searched with a modified BLAST considering both sequence and structure, enabling rapid taxon sampling. Novel sequences can be annotated using the HMM based approach and modelled according to multiple template structures. Sequences can be searched for known and newly identified motifs. Together, the database and the web server build an exhaustive resource for ITS2 based phylogenetic analyses.
Keywords:Base Sequence, Computational Biology, Fungal Genome, Fungi, Genetic Databases, Information Storage and Retrieval, Internet, Molecular Sequence Data, Nucleic Acid Databases, Nucleic Acid Sequence Homology, Plant Genome, Plants, Ribosomal Spacer DNA, Software, Tertiary Protein Structure
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press
Volume:38
Number:Suppl 1
Page Range:D275-D279
Date:January 2010
Official Publication:https://doi.org/10.1093/nar/gkp966
PubMed:View item in PubMed

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