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Spatial and temporal heterogeneity in high-grade serous ovarian cancer: a phylogenetic analysis

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Item Type:Article
Title:Spatial and temporal heterogeneity in high-grade serous ovarian cancer: a phylogenetic analysis
Creators Name:Schwarz, R.F. and Ng, C.K.Y. and Cooke, S.L. and Newman, S. and Temple, J. and Piskorz, A.M. and Gale, D. and Sayal, K. and Murtaza, M. and Baldwin, P.J. and Rosenfeld, N. and Earl, H.M. and Sala, E. and Jimenez-Linan, M. and Parkinson, C.A. and Markowetz, F. and Brenton, J.D.
Abstract:Background: The major clinical challenge in the treatment of high-grade serous ovarian cancer (HGSOC) is the development of progressive resistance to platinum-based chemotherapy. The objective of this study was to determine whether intra-tumour genetic heterogeneity resulting from clonal evolution and the emergence of subclonal tumour populations in HGSOC was associated with the development of resistant disease. Methods and Findings: Evolutionary inference and phylogenetic quantification of heterogeneity was performed using the MEDICC algorithm on high-resolution whole genome copy number profiles and selected genome-wide sequencing of 135 spatially and temporally separated samples from 14 patients with HGSOC who received platinum-based chemotherapy. Samples were obtained from the clinical CTCR-OV03/04 studies, and patients were enrolled between 20 July 2007 and 22 October 2009. Median follow-up of the cohort was 31 mo (interquartile range 22-46 mo), censored after 26 October 2013. Outcome measures were overall survival (OS) and progression-free survival (PFS). There were marked differences in the degree of clonal expansion (CE) between patients (median 0.74, interquartile range 0.66-1.15), and dichotimization by median CE showed worse survival in CE-high cases (PFS 12.7 versus 10.1 mo, p = 0.009; OS 42.6 versus 23.5 mo, p = 0.003). Bootstrap analysis with resampling showed that the 95% confidence intervals for the hazard ratios for PFS and OS in the CE-high group were greater than 1.0. These data support a relationship between heterogeneity and survival but do not precisely determine its effect size. Relapsed tissue was available for two patients in the CE-high group, and phylogenetic analysis showed that the prevalent clonal population at clinical recurrence arose from early divergence events. A subclonal population marked by a NF1 deletion showed a progressive increase in tumour allele fraction during chemotherapy. Conclusions: This study demonstrates that quantitative measures of intra-tumour heterogeneity may have predictive value for survival after chemotherapy treatment in HGSOC. Subclonal tumour populations are present in pre-treatment biopsies in HGSOC and can undergo expansion during chemotherapy, causing clinical relapse.
Keywords:Algorithms, Alleles, Disease-Free Survival, Genetic Variation, Glandular and Epithelial Neoplasms, Neoplasm DNA, Neoplasm Drug Resistance, Ovarian Neoplasms, Phylogeny, Platinum
Source:PLoS Medicine
Publisher:Public Library of Science
Page Range:e1001789
Date:24 February 2015
Additional Information:Erratum in: Nature 568(7751): E4.
Official Publication:https://doi.org/10.1371/journal.pmed.1001789
PubMed:View item in PubMed

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