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Isolation of a protein that is essential for the first step of nuclear protein import

Item Type:Article
Title:Isolation of a protein that is essential for the first step of nuclear protein import
Creators Name:Goerlich, D. and Prehn, S. and Laskey, R.A. and Hartmann, E.
Abstract:We have purified a cytosolic protein from Xenopus eggs that is essential for selective protein import into the cell nucleus. The purified protein, named importin, promotes signal-dependent binding of karyophilic proteins to the nuclear envelope. We have cloned, sequenced, and expressed a corresponding cDNA. Importin shows 44% sequence identity with SRP1p, a protein associated with the yeast nuclear pore complex. Complete, signal-dependent import into HeLa nuclei can be reconstituted by combining importin purified from Xenopus eggs or expressed in E. coli with Ran/TC4. Evidence for additional stimulatory factors is provided.
Keywords:Adenosine Triphosphate, Amino Acid Sequence, Base Sequence, Biological Transport, Cell Compartmentation, Cell Nucleus, Molecular Cloning, Cytosol, Complementary DNA, Hela Cells, Histocytochemistry, Karyopherins, Molecular Sequence Data, Nuclear Envelope, Nuclear Proteins, Ovum, Protein Binding, Recombinant Proteins, DNA Sequence Analysis, Amino Acid Sequence Homology, alpha Karyopherins, Animals, Xenopus
Publisher:Cell Press
Page Range:767-778
Date:2 December 1994
Official Publication:https://doi.org/10.1016/0092-8674(94)90067-1
PubMed:View item in PubMed

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