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Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution

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Item Type:Article
Title:Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution
Creators Name:Mauri, M. and Kirchner, M. and Aharoni, R. and Ciolli Mattioli, C. and van den Bruck, D. and Gutkovitch, N. and Modepalli, V. and Selbach, M. and Moran, Y. and Chekulaeva, M.
Abstract:Our current knowledge about the mechanisms of miRNA silencing is restricted to few lineages such as vertebrates, arthropods, nematodes and land plants. miRNA-mediated silencing in bilaterian animals is dependent on the proteins of the GW182 family. Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria.
Keywords:Cnidaria, Plants, Repression, RNA, Messenger, Tryptophan, MicroRNA, Animals, Nematostella
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press (U.K.)
Volume:45
Number:2
Page Range:938-950
Date:6 September 2016
Official Publication:https://doi.org/10.1093/nar/gkw792
PubMed:View item in PubMed

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