TLR2 controls random motility, while TLR7 regulates chemotaxis of microglial cells via distinct pathways
Item Type: | Article |
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Title: | TLR2 controls random motility, while TLR7 regulates chemotaxis of microglial cells via distinct pathways |
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Creators Name: | Ifuku, M. and Buonfiglioli, A. and Jordan, P. and Lehnardt, S. and Kettenmann, H. |
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Abstract: | Microglial cells are the pathologic sensor of the brain, and any pathologic event triggers microglial activation, which involves migration of these cells to a lesion site. Employing different migration assays, we show that ligands for toll-like receptor (TLR) 2 stimulate random motility, while TLR7 ligands are chemoattractants. The subtype specificity of the TLR ligands was verified by using different TLR-deficient (TLRKO) mouse lines. PI3K and Rac inhibition impairs both TLR2- and TLR7-stimulated microglial migration. In contrast, Akt phosphorylation is only required for the TLR2-, but not for the TLR7-stimulated pathway. Interestingly, P2Y12 receptor signaling is involved in the TLR2 activation-induced microglial migration but not TLR7. Furthermore, TLR7 mRNA expression is down-regulated by TLR2 and TLR7 activation. We conclude that TLRs control the migratory behavior of microglia in a distinct manner. |
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Keywords: | Toll-Like Receptor, Migration, Chemotaxis, PI3K, Akt, Rac, Animals, Mice |
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Source: | Brain Behavior and Immunity |
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ISSN: | 0889-1591 |
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Publisher: | Elsevier / Academic Press |
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Volume: | 58 |
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Page Range: | 338-347 |
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Date: | November 2016 |
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Official Publication: | https://doi.org/10.1016/j.bbi.2016.08.003 |
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PubMed: | View item in PubMed |
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