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TLR2 controls random motility, while TLR7 regulates chemotaxis of microglial cells via distinct pathways

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Item Type:Article
Title:TLR2 controls random motility, while TLR7 regulates chemotaxis of microglial cells via distinct pathways
Creators Name:Ifuku, M. and Buonfiglioli, A. and Jordan, P. and Lehnardt, S. and Kettenmann, H.
Abstract:Microglial cells are the pathologic sensor of the brain, and any pathologic event triggers microglial activation, which involves migration of these cells to a lesion site. Employing different migration assays, we show that ligands for toll-like receptor (TLR) 2 stimulate random motility, while TLR7 ligands are chemoattractants. The subtype specificity of the TLR ligands was verified by using different TLR-deficient (TLRKO) mouse lines. PI3K and Rac inhibition impairs both TLR2- and TLR7-stimulated microglial migration. In contrast, Akt phosphorylation is only required for the TLR2-, but not for the TLR7-stimulated pathway. Interestingly, P2Y12 receptor signaling is involved in the TLR2 activation-induced microglial migration but not TLR7. Furthermore, TLR7 mRNA expression is down-regulated by TLR2 and TLR7 activation. We conclude that TLRs control the migratory behavior of microglia in a distinct manner.
Keywords:Toll-Like Receptor, Migration, Chemotaxis, PI3K, Akt, Rac, Animals, Mice
Source:Brain Behavior and Immunity
ISSN:0889-1591
Publisher:Elsevier / Academic Press (U.S.A.)
Volume:58
Page Range:338-347
Date:November 2016
Official Publication:https://doi.org/10.1016/j.bbi.2016.08.003
PubMed:View item in PubMed

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