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reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells

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Item Type:Article
Title:reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells
Creators Name:Kinkley, S. and Helmuth, J. and Polansky, J.K. and Dunkel, I. and Gasparoni, G. and Froehler, S. and Chen, W. and Walter, J. and Hamann, A. and Chung, H.R.
Abstract:The combinatorial action of co-localizing chromatin modifications and regulators determines chromatin structure and function. However, identifying co-localizing chromatin features in a high-throughput manner remains a technical challenge. Here we describe a novel reChIP-seq approach and tailored bioinformatic analysis tool, normR that allows for the sequential enrichment and detection of co-localizing DNA-associated proteins in an unbiased and genome-wide manner. We illustrate the utility of the reChIP-seq method and normR by identifying H3K4me3 or H3K27me3 bivalently modified nucleosomes in primary human CD4(+) memory T cells. We unravel widespread bivalency at hypomethylated CpG-islands coinciding with inactive promoters of developmental regulators. reChIP-seq additionally uncovered heterogeneous bivalency in the population, which was undetectable by intersecting H3K4me3 and H3K27me3 ChIP-seq tracks. Finally, we provide evidence that bivalency is established and stabilized by an interplay between the genome and epigenome. Our reChIP-seq approach augments conventional ChIP-seq and is broadly applicable to unravel combinatorial modes of action.
Keywords:Data Processing, Histone Post-Translational Modifications, Immunochemistry, Statistical Methods
Source:Nature Communications
Publisher:Nature Publishing Group
Page Range:12514
Date:17 August 2016
Official Publication:https://doi.org/10.1038/ncomms12514
PubMed:View item in PubMed

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