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Role of cystathionine gamma-lyase in immediate renal impairment and inflammatory response in acute ischemic kidney injury

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Item Type:Article
Title:Role of cystathionine gamma-lyase in immediate renal impairment and inflammatory response in acute ischemic kidney injury
Creators Name:Markó, L. and Szijártó, I.A. and Filipovic, M.R. and Kaßmann, M. and Balogh, A. and Park, J.K. and Przybyl, L. and N'diaye, G. and Krämer, S. and Anders, J. and Ishii, I. and Müller, D.N. and Gollasch, M.
Abstract:Hydrogen sulfide (H2S) is known to act protectively during renal ischemia/reperfusion injury (IRI). However, the role of the endogenous H2S in acute kidney injury (AKI) is largely unclear. Here, we analyzed the role of cystathionine gamma-lyase (CTH) in acute renal IRI using CTH-deficient (Cth(-/-)) mice whose renal H2S levels were approximately 50% of control (wild-type) mice. Although levels of serum creatinine and renal expression of AKI marker proteins were equivalent between Cth(-/-) and control mice, histological analysis revealed that IRI caused less renal tubular damage in Cth(-/-) mice. Flow cytometric analysis revealed that renal population of infiltrated granulocytes/macrophages was equivalent in these mice. However, renal expression levels of certain inflammatory cytokines/adhesion molecules believed to play a role in IRI were found to be lower after IRI only in Cth(-/-) mice. Our results indicate that the systemic CTH loss does not deteriorate but rather ameliorates the immediate AKI outcome probably due to reduced inflammatory responses in the kidney. The renal expression of CTH and other H2S-producing enzymes was markedly suppressed after IRI, which could be an integrated adaptive response for renal cell protection.
Keywords:Acute Kidney Injury, Cystathionine Gamma-Lyase, Hydrogen Sulfide, Kidney, RNA, Messenger, Animals, Mice
Source:Scientific Reports
ISSN:2045-2322
Publisher:Nature Publishing Group (U.K.)
Volume:6
Page Range:27517
Date:8 June 2016
Official Publication:https://doi.org/10.1038/srep27517
PubMed:View item in PubMed

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