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Hyper-activation of ATM upon DNA-PKcs inhibition modulates p53 dynamics and cell fate in response to DNA damage

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Item Type:Article
Title:Hyper-activation of ATM upon DNA-PKcs inhibition modulates p53 dynamics and cell fate in response to DNA damage
Creators Name:Finzel, A. and Grybowski, A. and Strasen, J. and Cristiano, E. and Loewer, A.
Abstract:A functional DNA damage response is essential for maintaining genome integrity in the presence of DNA double strand breaks. It is mainly coordinated by the kinases ATM, ATR and DNA-PKcs, which control the repair of broken DNA strands and relay the damage signal to the tumor suppressor p53 to induce cell cycle arrest, apoptosis or senescence. Although many functions of the individual kinases have been identified, it remains unclear how they act in concert to ensure faithful processing of the damage signal. Using specific inhibitors and quantitative analysis at the single cell level, we systematically characterize the contribution of each kinase for regulating p53 activity. Our results reveal a new regulatory interplay, where loss of DNA-PKcs function leads to hyper-activation of ATM and amplification of the p53 response, sensitizing cells for damage-induced senescence. This interplay determines the outcome of treatments regimens combining irradiation with DNA-PKcs inhibitors in a p53-dependent manner.
Keywords:A549 Cells, Apoptosis, Ataxia Telangiectasia Mutated Proteins, Cell Cycle Proteins, DNA, DNA Damage, DNA Repair, DNA-Activated Protein Kinase, DNA-Binding Proteins, Double-Stranded DNA Breaks, MCF-7 Cells, Nuclear Proteins, Phosphorylation, Protein-Serine-Threonine Kinases, Signal Transduction, Tumor Cell Line, Tumor Suppressor Protein p53, Tumor Suppressor Proteins
Source:Molecular Biology of the Cell
Publisher:American Society for Cell Biology
Page Range:2360-2367
Date:1 August 2016
Official Publication:https://doi.org/10.1091/mbc.E16-01-0032
PubMed:View item in PubMed

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