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Isolation of eosinophils from the lamina propria of the murine small intestine

Item Type:Article
Title:Isolation of eosinophils from the lamina propria of the murine small intestine
Creators Name:Berek, C. and Beller, A. and Chu, V.T.
Abstract:Only recently has it become apparent that eosinophils play a crucial role in mucosal immune homeostasis. Although eosinophils are the main cellular component of the lamina propria of the gastrointestinal tract, they have often been overlooked because they express numerous markers, which are normally used to characterize macrophages and/or dendritic cells. To study their function in mucosal immunity, it is important to isolate them with high purity and viability. Here, we describe a protocol to purify eosinophils from the lamina propria of the murine small intestine. The method involves preparation of the small intestine, removal of epithelial cells and digestion of the lamina propria to release eosinophils. A protocol to sort eosinophils is included.
Keywords:Eosinophils, Lamina Propria, Small Intestine, Epithelial Cells, Peyer's Patches, Digestion, Animals, Mice
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Gastrointestinal physiology and diseases : methods and protocols
ISSN:1064-3745
ISBN:978-1-4939-3603-8
Publisher:Springer / Humana Press (U.S.A.)
Volume:1422
Page Range:213-221
Date:2016
Official Publication:https://doi.org/10.1007/978-1-4939-3603-8_20
PubMed:View item in PubMed

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