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Combined fluorescence and phosphorescence lifetime imaging

Item Type:Article
Title:Combined fluorescence and phosphorescence lifetime imaging
Creators Name:Shcheslavskiy, V.I. and Neubauer, A. and Bukowiecki, R. and Dinter, F. and Becker, W.
Abstract:We present a lifetime imaging technique that simultaneously records the fluorescence and phosphorescence lifetime images in confocal laser scanning systems. It is based on modulating a high-frequency pulsed laser synchronously with the pixel clock of the scanner, and recording the fluorescence and phosphorescence signals by multidimensional time-correlated single photon counting board. We demonstrate our technique on the recording of the fluorescence/phosphorescence lifetime images of human embryonic kidney cells at different environmental conditions.
Source:Applied Physics Letters
ISSN:0003-6951
Publisher:American Institute of Physics (U.S.A.)
Volume:108
Number:9
Page Range:091111
Date:3 March 2016
Official Publication:https://doi.org/10.1063/1.4943265

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