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Current approaches toward quantitative mapping of the interactome

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Item Type:Review
Title:Current approaches toward quantitative mapping of the interactome
Creators Name:Buntru, A. and Trepte, P. and Klockmeier, K. and Schnoegl, S. and Wanker, E.E.
Abstract:Protein-protein interactions (PPIs) play a key role in many, if not all, cellular processes. Disease is often caused by perturbation of PPIs, as recently indicated by studies of missense mutations. To understand the associations of proteins and to unravel the global picture of PPIs in the cell, different experimental detection techniques for PPIs have been established. Genetic and biochemical methods such as the yeast two-hybrid system or affinity purification-based approaches are well suited to high-throughput, proteome-wide screening and are mainly used to obtain qualitative results. However, they have been criticized for not reflecting the cellular situation or the dynamic nature of PPIs. In this review, we provide an overview of various genetic methods that go beyond qualitative detection and allow quantitative measuring of PPIs in mammalian cells, such as dual luminescence-based co-immunoprecipitation, Foerster resonance energy transfer or luminescence-based mammalian interactome mapping with bait control. We discuss the strengths and weaknesses of different techniques and their potential applications in biomedical research.
Keywords:PPI Analysis, FRET, DULIP, FCCS, PLA, Interactome Mapping, BiFC, LUMIER, BRET, Quantification of Protein-Protein Interactions
Source:Frontiers in Genetics
Publisher:Frontiers Media SA
Page Range:74
Date:4 May 2016
Official Publication:https://doi.org/10.3389/fgene.2016.00074
PubMed:View item in PubMed

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