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The mRNA-bound proteome of the early fly embryo

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Official URL:https://doi.org/10.1101/gr.200386.115
PubMed:View item in PubMed
Creators Name:Wessels, H.H. and Imami, K. and Baltz, A.G. and Kolinksi, M. and Beldovskaya, A. and Selbach, M. and Small, S. and Ohler, U. and Landthaler, M.
Journal Title:Genome Research
Journal Abbreviation:Genome Res
Volume:26
Number:7
Page Range:1000-1009
Date:July 2016
Keywords:Developmental Gene Expression Regulation, Drosophila Proteins, Drosophila melanogaster, Embryonic Development, Messenger RNA, Protein Binding, Proteome, RNA-Binding Proteins, Animals
Abstract:Early embryogenesis is characterized by the maternal to zygotic transition (MZT), in which maternally deposited messenger RNAs are degraded while zygotic transcription begins. Before the MZT, post-transcriptional gene regulation by RNA-binding proteins (RBPs) is the dominant force in embryo patterning. We used two mRNA interactome capture methods to identify RBPs bound to polyadenylated transcripts within the first two hours of D. melanogaster embryogenesis. We identified a high-confidence set of 476 putative RBPs and confirmed RNA-binding activities for most of 24 tested candidates. Most proteins in the interactome are known RBPs or harbor canonical RBP features, but 99 exhibited previously uncharacterized RNA-binding activity. mRNA-bound RBPs and TFs exhibit distinct expression dynamics, in which the newly identified RBPs dominate the first two hours of embryonic development. Integrating our resource with in situ hybridization data from existing databases showed that mRNAs encoding RBPs are enriched in posterior regions of the early embryo, suggesting their general importance in posterior patterning and germ cell maturation.
ISSN:1088-9051
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Item Type:Article

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